Establishment of regeneration system of Chrysanthemum morifolium ziyan and niu 9722

The regeneration systems of Chrysanthemum morifolium cv. ziyan and C. morifolium cv. niu 9722 were established with the aseptic stems with axillary bud as explants and basic medium with different concentrations of 6BA and NAA. The results showed that the optimal sterilization method was 2% NaClO for 6 min for ziyan and 2% NaClO for 8 min for niu 9722. The optimal medium for adventitious bud induction from leaves of ziyan was MS+6BA 2.0 mgL-1+NAA 1.0 mgL-1 with the callus induction ratio of 100%, the adventitious bud differentiation ratio of 92.22% and the reproduction coefficient of 7.60. The optimal medium for adventitious bud induction from leaves of niu 9722 was MS+6BA 2.0 mgL-1+NAA 0.5 mgL-1 with the callus induction ratio of 100% and the adventitious bud differentiation ratio of 45.59%. The optimal medium for stem proliferation of niu 9722 was MS+6BA 2.0 mgL-1+NAA 0.5 mgL-1 with the average multiplication coefficient of 10.05. The optimal medium for rooting of ziyan was 1/2 MS+NAA 0.2 mgL-1 with the rooting rate of 100% and the average root number of 15.50. The optimal medium for rooting of niu 9722 was 1/2 MS+NAA 0.3 mgL-1 with the rooting rate of 100% and the average root number of 14.87. The transferred plants grew well with survival rate of 100%.