Transformation of Puccinellia chinampoensis PuP5CS gene into alfalfa with Agrobacteriummediated method

In order to cultivate resistance alfalfa (Medicago sativa), the plant expression vector pCAMBIA3300PuP5CS was constructed and transformed into alfalfa from cotyledon via agrobacterium mediated. The transformed plants were selected with 2.0 mgL-1 glufosinate ammonium and the ratio of transformation callus differentiation was 22.4%. Eleven transformed plants were identified as positive in the gene expression by PCR and RTPCR which suggested that the PuP5CS gene had been integrated into the genome of the regenerated plants.