Molecular characterization and efficacy evaluation of a transgenic corn event AG16

Glyphosate-resistant transgenic maize can significantly improve the efficiency of weed control. In previous studies, the newly glyphosate-tolerant gene G10evo was integrated into the genome of maize named Hi-Ⅱ by Agrobacterium-mediated transformation, and different glyphosate-tolerant maize events were obtained. In the study, transgenic maize AG16 was selected for molecular characterization by PCR, Western blot, Southern blot and ELISA. Southern blot analysis using probe for G10evo showed that T-DNA was integrated in a single site in the maize genome. Western blot analysis showed that G10evo protein was expressed in leaf, stem and root of AG16. ELISA assay showed that the expression level of G10evo protein in the young stem reached 9.975 μg·g-1 before glyphosate spraying. Glyphosate spray assay in the greenhouse showed that the AG16 is tolerant to glyphosate at concentrations up to 4 times of recommended field application level. Therefore, the transgenic maize AG16 has the potential of field application, which is expected to become completely independent research and independent intellectual property rights of domestic glyphosate-resistant corn.