Identification and expression pattern analysis under abiotic stresses of bZIP gene family in orchardgrass

As a cool-season forage grass, orchardgrass (Dactylis glomerata) can be affected by various abiotic stresses during its growth. The bZIP transcription factor is involved in multiple biological processes, playing a critical role in resisting to biotic and abiotic stresses. In this study, genome-wide identification and analysis of DgbZIP gene family was performed and its expression profile was explored using bioinformatics and quantitative real-time PCR (qRT-PCR) methods. A total of 103 DgbZIP genes were identified and classified into 11 subfamilies. In addition, members of the same subfamily share similar conserved motif and gene structure, and cis-acting elements closely related to stress were predicted. The results showed that members of the A, C, and S subfamilies responded strongly to drought and heat stresses and were involved in expression regulation. The results of this study provide a reference to further examine the molecular mechanisms of the DgbZIP gene response to adverse stresses.