Fingerprint construction and hybrid purity identification of spring rapeseed varieties using InDel markers

The parents of 15 spring rapeseed (Brassica napus) varieties widely cultivated in Qinghai Province were used as materials, and 13 polymorphic markers were selected from 190 pairs of InDel markers as core InDel markers. PCR amplification was performed on the test materials using these 13 pairs of markers, followed by genetic diversity analysis, cluster analysis, DNA fingerprint construction, and hybrid purity identification of the 15 test materials. The results showed that 48 loci were amplified by the 13 pairs of primers, with an average of 3.7 loci per primer pair. The average number of effective alleles was 6.39, the average genetic diversity was 0.38, and the average Shannon’s index was 2.09. At a genetic similarity coefficient of 0.58, the 15 test materials were divided into 3 categories, with Class C containing the most materials, accounting for 73% of the total. The purity identification results for the hybrid species indicated that the InDel marker identification results were 2–5.5 percentage points lower than the field fertility survey results. This study provides a theoretical basis for the purity identification of newly selected rapeseed varieties and other main and hybrid varieties in Qinghai Province in recent years.