Genetic diversity of Sophora alopecuroides germplasm resources based on RAPD analysis

Genetic diversity of Sophora alopecuroides germplasm resources was analyzed at DNA level by Random Amplified Polymorphic DNA (RAPD). A total of 314 DNA bands from 22 populations in different habitats were amplified with 31 reliable RAPD primers, of which 285 DNA bands (90.76%) were polymorphic. The Neis gene diversity (H) and Shannon information index (I) were 0.301 5 and 0.453 1, respectively. The genetic distances ranged from 0.114-0.709. The results of RAPD unweighted pairgroup method with arithmetic means (UPGMA) cluster and the principal component analysis (PCA) demonstrated that 22 S.alopecuroides populations were clustered into 6 groups, which means that the genetic differentiations exist among populations.