Effect of procreation endocrine regulation during embryo transfer in sheep
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Abstract
Fifteen German Mutton Merino (14 months, female), fifteen Borderdale (14 months, female) and eighteen Poll Dorset (14 months, female) were selected as donor and with CIDR+PMSG+FSH treatment, 124 SmallTailed Han sheep were selected as the recipient and with CIDR + PMSG treatment to study the changes of the progesterone (p4), estradiol (E2), FollicleStimulatingHormone (FSH) and luteinizinghormone (LH). The results showed that the donor sheep and the recipient sheep reached a very ideal estrus synchronization state respectively. The luteal number of German Mutton Merino (7.71) was significantly higher than that of Dorset (5.19, P0.05). The number of eggs (including zygote and nonzygote) got from German Mutton Merino (5.50) was significantly higher than that of Dorset (3.44, P0.05); the rate of embryos effective of German Mutton Merino, Borderdal and Dorset were 70.13%, 55.74% and 78.18% respectively, and Dorset was significantly higher than that of Borderdale (P0.05); German Mutton Merino, Borderdale, Dorset embryo transfer pregnancy rate was 55.26%, 54.17% and 42.31% respectively, German Mutton Merino was significantly higher than that of Dorset (P0.05). During treatment of donor and recipient procreation hormone dynamic variation, for on the 13th day, the recipient reached its serum p4 peak, the SmallTailed Han sheep were higher than those of German Mutton Merino, Borderdale and Dorset. The E2 in serum of Donor reached its peak on the 14th day. The SmallTailed Han sheep reached its peak on the 15th day, The FSH in serum of donor on the 13th day reached its peak and SmallTailed Han sheep reached the peak on the 14th day. The LH in serum of donor reached its peak on the 14 day and Smalltailed Han sheep serum LH reached its peak on the 15th day.
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