Induction of plantlets in Corydalis decumbens using tissue culture

Bud germination, proliferation and the plantlets were induced from tuber of Corydalis decumbens using tissue culture based on different concentrations of hormones, tuber cutting parts and different temperatures. The results showed that the best induced temperature was 16 ℃ for the sprouting of tubers, bud proliferation and growth of the tuber plantlets. The best medium for tuber sprouting was 1/2MS+2.5 mg·L-16-BA+0.1 mg·L-1NAA+0.5 mg·L-1GA3+3% sucrose+0.75% agar; the optimal medium for bud proliferation was 1/2MS+3.5 mg·L-16-BA+0.1 mg·L-1NAA+ 0.5 mg·L-1GA3+3% sucrose+0.75% agar. The tube with skin had good results regarding bud propagation, in which the average number of induced shoots was 3.71. The multiple sprounts growed vigorously and formed plantlet easily. The 1/2MS basic medium without any hormone was the optimal medium for plantlet proliferation.