Study on callus induction and plantlet regeneration of Ophiopogon japonicus

The differentiation of Ophiopogon japonicus callus from different explants with different medium was conducted. The result showed that the base part of young leaf was best for callus induction. and the induction rate was more than 89% in G3 medium (MS+KT 0.2 mg/L +2，4D 0.1 mg/L), and the differentiation rate could reach 59% in J1 medium (MS +BA 2.0 mg/L +NAA 0.2 mg/L).