Study on alkalinity resistance of alfalfa callus in mutagenic treatments

The stem of alfalfa（Medicago sativa）was used as the experimental material, the inducement of callus was conducted, and the callus of alfafa was treated by ultraviolet ray and NaN3. Callus in mutagenic treat and control were filtrated by proline and forced alkalinity stress by NaHCO3 and Na2CO3, then they were appraised by the alkalinity resistance physiological indexes such as peroxidase activity, soluble protein content and proline content. It was shown that all these three indexes in the treated callus were evidently higher than those in the control. So the method of ultraviolet ray and NaN3 could be used to improve the alkalinity resistance of alfalfa, and ultraviolet ray treatment had greater effect on the alkali resistance of alfalfa than NaN3 treatment.