A study on salt tolerance of T1 generation transgenic perennial ryegrass withDREB1A gene at germination period

Abstract： The seeds of transgenic perennial ryegrass (Lolium perenne) with DREB1A gene were treated with various concentrations of NaCl (0, 0.3%, 0.6%, 0.9%, 1.2% and 1.5%) to study physiological responses of transgenic plants under salt stresses. The results showed that the seed relative germination potential, relative germination rate, seedling height, leaf chlorophyll content, leaf water content and relative water content (RWC) decreased when NaCl concentration increased. There were significant differences between the transgenic group and control group. Variation of root length was not obvious．The leaf relative conductivity had a positive relationship with the increase of salt concentration and with significant differences between transgenic plants and control plants. The salt tolerance of transgenic plants improved compared to nontransgenic plants. Transgenic T1 generation seeds of perennial ryegrass with DREB1A gene can be used as seed resources for salt soil improvement.