Determination of Oxysophocarpine in different structures of Sophora viciifolia by HPLC

This paper established an analytical method to determine the content of oxysophocarpine in Sophora viciifolia by HPLC. ZORBAXC18（150 mm4.6 mm,5 m）Column and 0.04 mol/L H3PO4(pH=1.8)－CH3OH(90∶10) mobile phase were used. The identification wave length was 220 nm and column temperature was 20 ℃.The result showed that the buds were the main accumulation organs of oysophocarpine (2.114%).The contents of oxysophocarpine in other organs such as young fruit, seeds, flowers, leaves, one year growing stem and perennial stem was 1.735%、1.651%、1.615%、0.540%、0.244% and 0.045%, respectively. This result is helpful for S. viciifolia resources development and ecology protection.