Study on in vitro propagation of Alhagi sparsifolia cuttings
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Abstract
Using Alhagi sparsifolia cuttings as explants, the effects of different concentration of 6BA and NAA, LH, GA3 on in vitro propagation and the effects of NAA on seedling rooting were discussed. The results are as follows:1) with 0.1 mg/L NAA, reducing 6BA concentration was benefit to stem growth. On the contrary, it is benefit to differentiation of buds;2) LH is advantaged to seedling growth and differentiation of buds,MS + 0.5 mg/L 6BA + 0.1 mg/L NAA +1.5 g/L LH was the better induction medium. Propagation multiple was 6.8;3)The effect of GA3 on in vitro propagation of A. sparsifolia was not significant;4)1/2MS+ 0.1 mg/L NAA was the best medium for rooting, the rooting rate was 71.7%.
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