Determination of polyphenol content in chicory by FolinCiocalteu colorimetry

A quantitative determination method of polyphenol in chicory was studied by using FolinCiocalteu colorimetry. Using gallic acid as standard and the detection wavelength was 765 nm. The results showed that the standard curve for quantifying polyphenol exhibited a good linearity in the range of 0~5 gmL-1 (R2=0.999 7). Furthermore, stability, precision, repetition and recovery rate were assayed with relative standard deviation (RSD) as 0.23%～1.60%. The method is convenient, fast, exact, and reliable to determine the total polyphenol in chicory. According to the fitted linear regression equation, the quantitative determination of chicory organs was made, and polyphenol content of root, stem, leaf, flower and seed were 6.71, 24.00, 32.03, 20.34 and 10.17 mgg-1, respectively.