Cloning and structure feature of adenylate kinase gene from a degrading strain of chlorpyrifos

The adenylate kinase (ADK) gene was amplified from an isolated strain Pseudomonas stutzeri 75 with ability to degrade organophosphorus pesticides, chlorpyrifos. The sequence of ADK75 gene was 810 bp in length and contained completed open reading frame (ORF) of 648 bp which encoded a polypeptide of 215 amino acids. After comparing with known sequences deposited in the GenBank, the highest homology of ADK75 gene was 99.5 %. Furthermore, the secondary and tertiary structures of ADK75 protein were analyzed. A typical ADK structure was found in the P. stutzeri strain 75 with three subdomains, that was AMPbinding subdomain (AMPbd), ATPbinding subdomain (LID), and the core subdomain. There were 3-13 and 5-16 amino acids substitution in AMPbd and LID, respectively. These changes might infer to be correlated with the characteristics of P. stutzeri strain 75 taking the chlorpyrifos as exclusive carbohydrate sources.