Construction of fingerprinting map based on SSR for Medicago sativa Huaiyin

In the present study, the genetic diversity of alfalfa was investigated using SSR molecular makers and optimum number of samples was determined using different sampling strategies. Based on the optimum number of samples with the screened SSR primers, the fingerprint of Huaiyin alfalfa was built and the varieties of Huaiyin alfalfa were identified. Genomic DNA from 320 strains of Huaiyin alfalfa was set to six treatments (alfalfa DNA samples randomly obtained from a mixture of equal amount of 10, 20, 30, 40, 50 and 60 plants , respectively). Each treatment has eight repeats. Genetic diversity analysis was conducted using four pairs of SSR primers. The results showed that the genetic diversity index of the treatment of andom DNA mixture from 40 individuals was stable and the fingerprint of Huaiyin alfalfa can represent the genetic diversity of Huaiyin alfalfa groups. Fifteen alfalfa materials were tested with the treatment mentioned above and the results showed that the SSR fingerprinting could be used for the identification of varieties in Huaiyin alfalfa groups.