Effects of different methods on NDF and ADF of silage

Taking Van Soest as control, the ANKOM bag, CAU bag, and P2 Glass crucible filters were used to measure NDF and ADF of eight kinds of silages often using in the Inner Mongolia for determine the effect of the different filters and testing methods on neutral detergent fiber (NDF) and acid detergent fiber (ADF) content of silage. NDF was treated by amylase heatstable and ADF was measured by continuous washing. This study indicated that NDF and ADF measured by CAU bag and ANKOM bag were not significantly different but they were significantly lower than those measured by P2 Glass crucible (P0.05), especially NDF in TMR silage and ADF in wholeplant corn silage and TMR silage. Compared with control, the NDF of whole plant corn silage and TMR silage significantly decreased by adding high temperature amylase（P＜0.05）and continuous washing greatly impacted on ADF of alfalfa silage and Lespedeza hedysaroides silage.