Method for plant chromatin immunoprecipitation assay

The dynamic chromatin structure,and timely and proper regulation of genes played crucial roles in plant growth and development.It is important for understanding complex life process to study association between protein and DNA.The chromatin immunoprecipitation is widely used to explore in vivo interaction between proteins and DNA.In this paper,the efficient procedures were developed starting from isolation the nuclei,then formaldehyde crossing linking,and the details of six main steps for this assay were also provided.The different binding modes of H3K9Me in transposon At4g03870 between wild type Col-0 and mutant met1 were tested by chromatin immunoprecipitation.The results showed that the method worked well and was not only available for determining the association of proteins with specific gene,but also for genome-wide identification of protein-DNA binding site.